Recovery of protein from reverse micelles through gas hydrate formation

Authors
Noritomi, H Hidaka, Y Kato, S Nagahama, K
Citation
H. Noritomi et al., Recovery of protein from reverse micelles through gas hydrate formation, BIOTECH TEC, 13(3), 1999, pp. 181-183
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
3
Year of publication
1999
Pages
181 - 183
Database
ISI
SICI code
0951-208X(199903)13:3<181:ROPFRM>2.0.ZU;2-4
Abstract
The recovery of cytochrome c and ribonuclease A from di-2-ethylhexyl sodium sulfosuccinate (AOT) reverse micelles have been examined by the gas hydrat e formation. The recovery of cytochrome c depended upon the kind of gas and the water content (w(0) = [H2O]/[AOT]) of reverse micellar solution contai ning cytochrome c prepared. Recoveries of cytochrome c and ribonuclease A w ere more than 80%, when 1,1,1,2-tetrafluoroethane (TFE) was used as a hydra ting gas. The activity of cytochrome c recovered from reverse micelles was maintained perfectly.