On-line recovery of large molecules from mixtures using reciprocating sizeexclusion chromatography

Blue Dextran, a standard large molecule, was successfully recovered on-line from the aqueous mixture solution with nickel nitrate using a novel recipr ocating size exclusion chromatography. After 7 cycles of repeating operatio ns of frontal mode, 70% of Blue Dextran in the feed was isolated as a pure solution. On-line recovery of large molecules from the mixture is an unusua l trial, comparing to the routine practice of filtration where small molecu le is isolated from the mixture.