Evidence for two candidate tumour suppressor loci on chromosome 9q in transitional cell carcinoma (TCC) of the bladder but no homozygous deletions inbladder tumour cell lines
Aag. Van Tilborg et al., Evidence for two candidate tumour suppressor loci on chromosome 9q in transitional cell carcinoma (TCC) of the bladder but no homozygous deletions inbladder tumour cell lines, BR J CANC, 80(3-4), 1999, pp. 489-494
The most frequent genetic alterations in transitional cell carcinoma (TCC)
of the bladder involve loss of heterozygosity (LOH) on chromosome 9p and 9q
. The LOH on chromosome 9p most likely targets the CDKN2 locus, which is in
activated in about 50% of TCCs. Candidate genes that are the target for LOH
on chromosome 9q have yet to be identified. To narrow the localization of
one or more putative tumour suppressor genes on this chromosome that play a
role in TCC of the bladder, we examined 59 tumours with a panel of microsa
tellite markers along the chromosome. LOH was observed in 26 (44%) tumours.
We present evidence for two different loci on the long arm of chromosome 9
where potential tumour suppressor genes are expected. These loci are delin
eated by interstitial deletions in two bladder tumours. Our results confirm
the results of others and contribute to a further reduction of the size of
these regions, which we called TCC1 and TCC2. These regions were examined
for homozygous deletions with EST and STS markers. No homozygous deletions
were observed in 17 different bladder tumour cell lines.