Effects of the endogeneous cannabinoid, anandamide, on neuronal activity in rat hippocampal slices

1 The arachidonic acid derivative arachidonylethanolamide (anandamide) is a n endogeneous ligand of cannabinoid receptors that induces pharmacological actions similar to those of cannabinoids such as Delta(9)-tetrahydrocannabi nol (THC). We examined whether anandamide can influence excessive neuronal activity by investigating stimulation-induced population spikes and epilept iform activity in rat hippocampal slices. For this purpose, the effects of anandamide were compared with those of the synthetic cannabinoid agonist WI N 55,212-2 and its inactive S(-)-enantiomer WIN 55,212-3. 2 Both anandamide (1 and 10 mu M) and WIN 55,212-2 (0.1 and 1 mu M) decreas ed the amplitude of the postsynaptic population spike and the slope of the field excitatory postsynaptic potential (field e.p.s.p.) without affecting the presynaptic fibre spike of the afferents. At a concentration of 1 mu M, WIN 55,212-2 completely suppressed the postsynaptic spike, whereas the S(- )-enantiomer WIN 55,212-3 produced only a slight depression. The CBI recept or antagonist SR 141716 blocked the inhibition evoked by the cannabinoids. SR 141716 had a slight facilitatory effect on neuronal excitability by itse lf. 3 Anandamide shifted the input-output curve of the postsynaptic spike and t he field e.p.s.p. to the right and increased the magnitude of paired-pulse facilitation indicating a presynaptic mechanism of action. 4 Anandamide and WIN 55,212-2, but not WIN 55,212-3, attenuated both stimul us-triggered epileptiform activity in CA1 elicited by omission of Mg2+ and spontaneously occurring epileptiform activity in CA3 elicited by omission o f Mg2+ and elevation of K+ to 8 mM. The antiepileptiform effect of these ca nnabinoids was blocked by SR 141716. 5 In conclusion, cannabinoid receptors of the CB1 type as well as their end ogeneous ligand, anandamide, are involved in the control of neuronal excita bility, thus reducing excitatory neurotransmission at a presynaptic site, a mechanism which might be involved in the prevention of excessive excitabil ity leading to epileptiform activity.