Translocation (11;14)(q13;q32) and preferential involvement of chromosomes1, 2, 9, 13, and 17 in mantle cell lymphoma

We have studied 13 cases of histologically confirmed mantle cell lymphomas (MCL) combining cytological-immunological features with conventional cytoge netics and in situ hybridization (ISH) techniques. Peripheral blood smears and lymph node biopsies expressed the typical mantle zone pattern with a B- cell phenotype. Most of the cases (11 of 13) had lymphomatous cells in the peripheral blood. Chromosome analysis was carried out on lymphoid cells fro m peripheral blood and/or lymph node biopsies. Phytohemagglutinin (PHA) and phorbol 12-myristate 13 acetate (TPA) were used as mitogens. Biotin-labele d libraries of whole chromosomes implicated in complex karyotypes were used to improve their interpretation. Clonal chromosome abnormalities were foun d in 10 of 13 patients (77%); 7 of these had a complex abnormality. The mos t frequent recurrent structural abnormalities were: t(11;14)(q13;q32), invo lvement of chromosome 1 (der[1], del[1], dup[1]), chromosome 2 (del[2], der [2]), chromosome 9 (der[9], -9), chromosome 13 (add[13], t[13q]), and chrom osome 17 (add[17], der[17], t[17q]). The most frequent numerical abnormalit ies it ere monosomy 21 and loss of the Y chromosome. (C) Elsevier Science I nc., 1999. All rights reserved.