The CD95/CD95 ligand (CD95L)system plays an important role in the induction
of lymphoid apoptosis and has been implicated in the suppression of immune
responses. In this system, two murine CD95L-transfected renca clones and a
control renca clone transfected only with the vector were implanted into t
he subcapsule of the left kidney of Balb/c and Balb/c nude mice. Both CD95L
-expressing and control renca clones formed macroscopic tumors in all of th
e Balb/c and Balb/c nude hosts 14 days after implantation. Growth of tumors
of murine CD95L-transfected renca cells was significantly better than that
of control renca cells in Balb/c mice, while the growth advantage of CD95L
transfectants was not observed in Balb/c nude mice. Lymphocytes underwent
apoptosis mainly in the periphery of the CD95L-expressing tumors but not in
control tumors grown in Balb/c mice, while lymphocytes undergoing apoptosi
s were not observed in CD95L-expressing tumors or in control tumors grown i
n Balb/c nude mice. Neutrophilic recruitment was rarely observed in CD95L-e
xpressing or control tumors. CD95L ex; pressed on renca cells possibly supp
ressed immune responses against renca tumors by inducing apoptosis of the i
nfiltrating lymphocytes. However, CD95L-expressing renca cells did not form
tumors in the renal subcapsule of allogeneic C3H/HeJ mice.