Cytogenetic alignment of the bovine chromosome 13 genome map by fluorescence in-situ hybridization of human chromosome 10 and 20 comparative markers

A bovine bacterial artificial chromosome (BAC) library was screened for the prescence of six genes (IL2RA, VIM, THBD, PLC-II, CSNK2A1 and TOP1) previo usly assigned to human chromosomes 10 or 20 (HSA10 or HSA20). Four of the g enes were found represented in the bovine BAC library by at least one clone . The identified BAC clones were used as probes in single-color fluorescenc e in-situ hybridization (FISH) to determine the chromosomal band location o f each gene. As predicted by the human/bovine comparative map and comparati ve chromosome painting analysis, the four genes mapped to bovine chromosome 13 (BTA13). Dual-color FISH was then used to integrate these four type I m arkers into the existing BTA13 genome map. These FISH results anchor the BT A13 genome map from bands 14-23, and confirm the presence of a conserved HS A10 homologous synteny group on BTA13 centromeric to a HSA20 homologous seg ment.