Bartonella henselae was recovered from the conjunctival scraping of a 38-ye
ar-old woman who presented with a 2-week history of tender preauricular lym
phadenopathy and a 1-day history of a red left eye. Dry adherent colonies w
ere observed on agar plates at 21 days of incubation, and the isolate was i
dentified through conventional and molecular tests. Polymerase chain reacti
on (PCR) amplification of a specific region of the 16S rRNA gene and confir
mation by a separate PCR reaction with hybridization of the product with a
B. henselae-specific probe confirmed the isolate as B. henselae. This is th
e first reported isolation of the causative agent of cat scratch disease fr
om ocular tissue in a patient with Parinaud's oculoglandular syndrome.