E. Held et T. Agner, Comparison between 2 test models in evaluating the effect of a moisturizeron irritated human skin, CONTACT DER, 40(5), 1999, pp. 261-268
The purpose of the present study was to compare 2 experimental models of mo
isturizer efficacy on the recovery of irritated skin on the hands and the v
olar forearms. 12 healthy volunteers had their hands immersed in sodium lau
ryl sulfate (SLS) 10 min 2x daily for 2 days, and at the same time the volu
nteers had patch tests with SLS (0.125%, 0.25% and 0.5%) applied on their f
orearms for 24 h. After irritation of the skin, the volunteers had a moistu
rizer applied on one arm/hand 3x daily for the following 9 days. The other
arm/hand served as untreated control. Evaluation was done on days (D) 1, 3,
5, 8 and 12 by transepidermal water loss, electrical capacitance, laser Do
ppler flowmetry and DermaSpectrometry. Both models were found useful, and t
he moisturizer was found to accelerate regeneration of the skin barrier fun
ction in both the hands (D8, p<0.05) and the volar forearms (0.5% SLS, D5 a
nd D8, p<0.01). When the forearm model is used in the present set-up, a rel
atively high concentration of SLS (>0.25%) should be used and evaluation me
asurements are best performed on D5-D8. The forearm model proved reliable a
nd easy to handle and we suggest that this model is used in future studies
on moisturizer evaluation.