Objective: Multiple endocrine neoplasia type 1 (MEN 1) is an autosomal domi
nant inherited disorder characterised by the combined occurrence of parathy
roid, endocrine pancreas and anterior pituitary tumours. The gene responsib
le for MEN 1, the menin gene, a putative tumour-suppressor gene located on
human chromosome 11q13, has been cloned. To investigate the role of the men
in gene in sporadic anterior pituitary tumorigenesis, its mRNA was assessed
in a group of pituitary tumours.
Methods: Menin gene expression, along with glyceraldehyde phosphate dehydro
genase (GAPDH) gene expression, has been studied in a group of normal pitui
taries and in 23 pituitary tumours not associated with the MEN 1 syndrome.
The pituitary tumours included 4 prolactinomas, 11 growth-hormone-secreting
tumours and 8 non-functional tumours. Total RNA was extracted from the nor
mal pituitaries and tumours, and cDNA was synthesised with standard reverse
transcriptase methods. Duplex polymerase chain reaction (PCR) was standard
ised in order to quantify the expression of the menin gene using intron-spa
nning primers across exons 9 and 10 in relation to thc 'house-keeping' gene
GAPDH. The PCR products were separated on agarose gel and densitometric an
alysis of the bands allowed semi-quantification.
Results: There was no evidence for a change in menin gene expression in any
of the pituitary tumours when compared with normal pituitaries.
Conclusions: These studies complement previous work on mutational analysis,
and do not suggest a major role for the menin suppressor gene in sporadic
pituitary tumorigenesis.