J. Martin-sanchez et P. Garcia-fernandez, Theileria annulata: Genetic characterization of Spanish isolates by isoenzyme electrophoresis and random amplified polymorphic DNA, EXP PARASIT, 92(1), 1999, pp. 57-63
Isoenzyme electrophoresis and RAPD techniques were used to study the geneti
c polymorphism of different Spanish isolates of Theileria annulata in the s
chizont and piroplasm stages. Enzyme activity attributable to the parasite
was detected in only 5 of the 13 loci studied with isoenzyme electrophoresi
s. Of these, differences between the cell lines studied were found only in
the loci GPI, ICD, and RI. Only 6 of the 11 primers used in the RAPD genera
ted reproducible genomic DNA fingerprints. None of the amplification produc
ts generated using primers ILO 509, ILO 525, ILO 872, and no 875 hybridized
with DNA of the bovine cell line not infected by T. annulata, BL20, indica
ting that this technique can be used with either piroplasm DNA or DNA from
parasite schizonts after first passing it through DEAE cellulose columns. T
he results obtained with both characterization techniques demonstrate a mod
erate degree of polymorphism among the Spanish isolates of this protozoan.
(C) 1999 Academic Press.