Monitoring the conjugal transfer of plasmid RP4 in activated sludge and insitu identification of the transconjugants

Authors
Geisenberger, O Ammendola, A Christensen, BB Molin, S Schleifer, KH Eberl, L
Citation
O. Geisenberger et al., Monitoring the conjugal transfer of plasmid RP4 in activated sludge and insitu identification of the transconjugants, FEMS MICROB, 174(1), 1999, pp. 9-17
Citations number
19
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097 → ACNP
Volume
174
Issue
1
Year of publication
1999
Pages
9 - 17
Database
ISI
SICI code
0378-1097(19990501)174:1<9:MTCTOP>2.0.ZU;2-E
Abstract
A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to mo nitor the conjugative transfer of the plasmid from a Pseudomonas putida don or strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4x10(-6) to 1x10(-5) transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targe ted oligonucleotides was used to phylogenetically affiliate the bacteria th at had received the plasmid. (C) 1999 Federation of European Microbiologica l Societies. Published by Elsevier Science B.V. All rights reserved.