Mh. Nathanson et al., Communication via gap junctions modulates bile secretion in the isolated perfused rat liver, GASTROENTY, 116(5), 1999, pp. 1176-1183
Background & Aims: Bile secretion is regulated in part by adenosine 3',5'-c
yclic monophosphate (cAMP) and cytosolic Ca2+ (Ca-i(2+)). Hormone receptors
that link to these second messengers are not uniformly distributed across
the hepatic lobule, but both cAMP and Ca-i(2+) cross gap junctions, so we t
ested whether gap junctional communication plays a role in changes in bile
flow induced by the activation of these receptors,
Methods: cAMP levels in isolated perfused rat livers were increased by usin
g glucagon, because glucagon receptors are predominantly on pericentral hep
atocytes, or by using dibutyryl cAMP, which acts on hepatocytes throughout
the hepatic lobule, Ca-i(2+) concentration was increased by using vasopress
in, because V-1a receptors are most heavily expressed on pericentral hepato
cytes, or by using 2,5-di(tert-butyl)-1,4-benzo-hydroquinone (t-BuBHQ), whi
ch increases the Ca-i(2+) concentration in hepatocytes throughout the hepat
ic lobule. We used 18 alpha-glycyrrhetinic acid (alpha GA) to block gap jun
ction conductance, which was assessed by fluorescence recovery after photob
leaching.
Results: alpha GA blocked fluorescence recovery after photobleaching withou
t altering the basal rate of bile flow, Glucagon and dibutyryl cAMP increas
ed bile flow; alpha GA blocked the glucagon-induced increase but not that i
nduced by dibutyryl cAMP, Vasopressin and t-BuBHQ decreased bile flow; alph
a GA exacerbated the decrease induced by vasopressin but not by t-BuBHQ,
Conclusions: Glucagon and vasopressin modulate bile flow in a manner that d
epends in part on gap junctional communication, even though the two hormone
s activate second messengers with opposing effects on bile flow. The organi
zation of second messenger signals across the hepatic lobule may be an impo
rtant component of hormonal regulation of bile secretion.