Characteristics of microscopic structures in bone cross sections tarry esse
ntial clues in age determination in forensic science and in the study of ag
e-related bone developments and bone diseases. Analysis of bone cross secti
ons represents a major area of research in bone biology. However, tradition
al approaches in bone biology have relied primarily on manual processes wit
h very limited number of bone samples. As a consequence, it is difficult to
reach reliable and consistent conclusions, Tn this paper we present an ima
ge processing system that uses microstructural and relational knowledge pre
sent in the bone cross section for bone image segmentation, This system aut
omates the bone image analysis process and is able to produce reliable resu
lts based on quantitative measurements from a large number of bone images.
As a result, using large databases of bone images to study the correlation
between bone structural features and age-related bone developments becomes
feasible.