Effect of sephadex filtration of buffalo bull semen on freezability and sperm penetration into homologous cervical mucus

Semen of 4 healthy fertile buffalo bulls (48 collections) was frozen in tri s-egg yolk glycerol extender before and after filtration through sephadex G 75-120 to investigate the effect of filtration on freezability and sperm p enetration into homologous cervical mucus. Cervical mucus of 10 normal cycl ing buffaloes in standing heat was pooled to remove the effect of mucus qua lity on sperm penetration. Filtration of semen through sephadex column resu lted in significantly (P<0.05) better maintenance of sperm motility, live s perm count, intact acrosomes and sperm morphology. Sephadex filtered frozen semen showed significantly better sperm penetration into homologous cervic al mucus compared to unfiltered semen. It is, thus, concluded that filtrati on of semen through sephadex column improves the quality of frozen semen.