Histoenzymic study was conducted for localization of phosphatases and acety
lcholinesterase in corpora lutea of 22 cyclic and 10 pregnant buffaloes. Th
e capsule and stroma were negative. Luteal cells were positive for alkaline
phosphatase (AKPase), acid phosphatase (ACPass), adenosine triphosphatase
(ATPase), glucose-6-phosphatase (G-6-Pase) and acetylcholinesterase (ACEase
). The activities were better demonstrable in developed corpora lutes and t
hat of pregnancy which reflect better physiological secretory activity at t
hese stages.