Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov.

The structural gene encoding the 16S rRNA of the new obligate intracellular organism presently designated WSU 86-1044(T) was sequenced and analysed to establish its phylogenetic relationships. The 16S rDNA sequence was most c losely related to those of chlamydial species, having 84.7-85.3% sequence s imilarity, while it had 72.4-73.2% similarity with rickettsia-like organism s. When the sequences of the four species of chlamydiae (Chlamydophila psit taci, Chlamydia trachomatis, Chlamydophila pneumoniae and Chlamydophila pec orum) were compared, they had > 93% sequence similarity indicating that WSU 86-1044(T) was not close enough to be in the same family as current Chlamy diaceae members. However, based on the 84.7-85.3% 16S rDNA sequence similar ity of WSU 86-1044(T) and other previously described characteristics, WSU 8 6-1044(T) belongs to a novel family within the order Chlamydiales; hence, t he proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp, n ov.