Rapid identification of hyperthermophilic methanococci isolated from deep-sea hydrothermal vents

Authors
Jeanthon, C L'Haridon, S Pradel, N Prieur, D
Citation
C. Jeanthon et al., Rapid identification of hyperthermophilic methanococci isolated from deep-sea hydrothermal vents, INT J SY B, 49, 1999, pp. 591-594
Citations number
27
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
00207713 → ACNP
Volume
49
Year of publication
1999
Part
2
Pages
591 - 594
Database
ISI
SICI code
0020-7713(199904)49:<591:RIOHMI>2.0.ZU;2-6
Abstract
16S rDNAs amplified by PCR from 22 hyperthermophilic methanococci isolated from deep-sea hydrothermal vents were compared with those of the six type s trains of the genus Methanococcus by RFLP analysis. Restriction fragments o btained with HaeIII enabled four of the type species to be distinguished. R estrictions with Hhal, BstUI and Mspl were necessary to differentiate Metha nococcus jannaschii and Methanococcus fervens. The results indicate that th e 16S rDNA PCR-RFLP method provides a rapid and reliable tool for the ident ification of newly isolated hyperthermophilic Methanococcus spp.