Human skeletal muscle HSP70 response to training in highly trained rowers

Previous studies have demonstrated exercise-induced heat shock protein 70 ( HSP70) in animals. The purpose of this study was to investigate human skele tal muscle HSP70 response to rowing training. Ten male rowers trained for 4 wk with different forms, durations, and intensities of exercise. Biopsy wa s performed in the right musculus vastus lateralis before training and at t he end of each week. HSP70 in 5 mu g of total protein from the muscle sampl e was determined by using Western blot and immunodetection with chemilumine scence technique, by means of laser densitometer referring to a series of k nown standard HSP70. Compared with pretraining (100%), HSP70 increased duri ng training (181, 405, 456, and 363% from the first to fourth training week , respectively) with the maximum HSP70 production at the end of second trai ning week. Thus HSP70 is induced in highly trained human muscle by long-ter m training.