Enzymatic synthesis of fructosyl glycerol

Authors
Gonzalez-Munoz, F Perez-Oseguera, A Cassani, J Jimenez-Estrada, M Vazquez-Duhalt, R Lopez-Munguia, A
Citation
F. Gonzalez-munoz et al., Enzymatic synthesis of fructosyl glycerol, J CARB CHEM, 18(3), 1999, pp. 275-283
Citations number
13
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CARBOHYDRATE CHEMISTRY
ISSN journal
07328303 → ACNP
Volume
18
Issue
3
Year of publication
1999
Pages
275 - 283
Database
ISI
SICI code
0732-8303(1999)18:3<275:ESOFG>2.0.ZU;2-Z
Abstract
The enzymatic synthesis of beta 2-2' and beta 2-1'-D- fructopyranosyl glyce rol was carried out with levansucrase from Bacillus circulans or B. subtili s, using sucrose as fructosyl donor and glycerol. The specificity and effic iency of the enzyme was modified by controlling both the water and the tota l substrate concentrations. The products were purified by HPLC and analyzed by H-1, C-13 NMR and GC-MS.