M. Obayashi et al., Determination of concentrations of flecainide in human serum by high-performance liquid chromatography on a fluorocarbon-bonded silica gel column, J CHROMAT B, 726(1-2), 1999, pp. 219-223
An optimized method for the determination of flecainide in serum is present
ed. Extraction using a solid-phase C-18 column and chromatography on a stab
ilized fluorocarbon-bonded silica gel column effectively separate flecainid
e from an internal standard (a positional isomer of flecainide). The HPLC a
pparatus and conditions were as follows: analytical column, Fluofix 120N: s
ample solvent, 20 mu l; column temperature, 40 degrees C; detector, Shimadz
u RF-5000 fluorescence spectrophotometer (excitation wavelength=300 nm, emi
ssion wavelength=370 mm); mobile phase, 0.06% phosphoric acid containing 0.
1% tetra-n-butyl ammonium bromide-acetonitrile (75:25, v/v); flow-rate, 1.0
ml/min. The standard curves for flecainide were linear in the concentratio
n range examined (10-2000 ng/ml). The regression equation was y=0.08+0.0078
x (r=0.9998). The minimum detectable amount of flecainide was approximately
5 ng/ml. In the within-day study, the precision coefficients of variation
were 2.66, 2.18, 2.54, 2.72, 2.88, 2.24, and 3.29% for the 10, 50, 100, 200
, 500, 1000, and 1500 ng/ml standards, respectively. The absolute recovery
rates of flecainide at each concentrations were 94-100%. The method describ
ed provides analytical sensitivity, specificity and reproducibility suitabl
e for both biomedical research and therapeutic drug monitoring. (C) 1999 El
sevier Science B.V. All rights reserved.