W. Engels et al., DIETARY MODULATION OF FATTY-ACID COMPOSITION OF MAST-CELL PHOSPHOLIPIDS DOES NOT AFFECT HISTAMINE-RELEASE INDUCED BY COMPOUND-48 80/, Inflammation research, 46(5), 1997, pp. 185-190
Objective and Design: In the present study we determined the extent to
which the degranulation process in mast cells was related to the fatt
y acid composition of membrane phospholipids. Material: Peritoneal mas
t cells were isolated from Wistar rats (3 groups of 18 animals each),
fed for 6 weeks diets which differed in their fatty acid compositions:
(i) genuine salmon oil, abundant in (n-3) fatty acids, (ii) sunflower
seed oil, rich in (n-6) fatty acids, particularly linoleic acid, and (
iii) hydrogenated coconut oil, rich in saturated fatty acids. Methods:
Mast cells (10(6)/ml) were stimulated with various concentrations of
the mast cell-degranulating agent, compound 48/80 (0.1-10 mu g/ml). Th
e extent of mast cell degranulation was quantified by determination of
histamine in the supernatants using HPLC techniques. Results: No diff
erences in compound 48/80-induced histamine release between the three
dietary groups for any of the concentrations of compound 48/80 tested
were found. Analysis of variance followed by Tukey's method for multip
le comparisons was used to evaluate the effect of changes in the dieta
ry fat type. Conclusion: These findings strongly suggest that in contr
ast to the formation of eicosanoids, the process of mast cell degranul
ation by a receptor-independent pathway is not controlled by the fatty
acid composition of membrane phospholipids.