Alteration in diastolic function following cardiac cryopreservation at subzero temperatures

Background: We have studied the alterations produced in the diastolic funct ion of the left ventricle after applying a protocol of cryopreservation at subzero temperatures. Methods: Isolated rabbit hearts and 5% polyethylene glycol (PM 4000) as the cryoprotective agent were used for the study. Results-Conclusions: Following cryopreservation we found a statistically si gnificant increase in systolic function. However, the diastolic function sh ows worsening, with a statistically significant increase (p < 0.05) in mean stiffness, decrease in differential stiffness, (p < 0.05) and upward and l eftward displacement of the diastolic pressure volume curve.