We introduce a new method to determine the 'lattice offset' or 'rigid-body
shift' between two crystals forming an interface. Our method relies on quan
titative evaluation of high-resolution transmission electron microscopy ima
ges. Employing the (001)-orientated interface between Al and MgAl2O4 in par
allel orientation as a model system we demonstrate that we can assess the i
nterface lattice offset with a precision in the picometre range.