Antimycobacterial triterpenes from Melia volkensii

In a bioassay-guided search for antimycobacterial compounds from higher pla nts, we have chemically investigated methanolic extracts of seeds of Melia volkensii. Chromatographic fractions provided two new euphane (20R)-type tr iterpenoids. The structures of the new compounds, 12 beta-hydroxykulactone (1) and 6 beta-hydroxykulactone (2), were elucidated by 1D and 2D NMR (C-13 , H-1, H-1-H-1 COSY, HMQC, HMBC, and NOESY spectra) and FABMS studies and s hown to be hydroxyl derivatives of kulactone (3). Also isolated was the kno wn kulonate (4). In a radiorespirometric bioassay against Mycobacterium tub erculosis, compounds 1, 2, and 4 exhibited minimum inhibitory concentration s of 16, 4, and 16 mu g/mL, respectively.