F. Mcgarry et al., The -308.1 polymorphism in the promoter region of the tumor necrosis factor gene is associated with ankylosing spondylitis independent of HLA-B27, J RHEUMATOL, 26(5), 1999, pp. 1110-1116
Objective. To investigate the allele frequencies of 6 polymorphic sites spa
nning the region of the genome close to the tumor necrosis factor (TNF) gen
e in a group of HLA-B27 positive patients with ankylosing spondylitis (AS)
in the West of Scotland.
Methods. One hundred sixty-seven patients with AS, 93 healthy controls, and
88 HLA-B27 positive healthy controls, all from the West of Scotland, were
typed by polymerase chain reaction (PCR) for 3 restriction fragment length
polymorphisms (RFLP) and 3 microsatellite polymorphic sites spanning the TN
F gene cluster. The distribution of these alleles was correlated with the p
resence of extraspinal manifestations such as peripheral joint disease and
uveitis.
Results. The frequency of the Nco-1.2 allele was significantly reduced in p
atients compared to the genetically unselected control population (p < 0.05
), but was no different from the MLA-B27 positive controls. However, the -3
08.1 allele was significantly increased in the patients compared to the HLA
-B27 positive controls (p < 0.03).
Conclusion. This study confirms the importance of correct matching in genet
ic analysis in disease association studies, and provides further evidence s
upporting the involvement of genes other than the MHC class I locus in the
pathogenesis and features of AS.