Periplakin and envoplakin are target antigens in canine and human paraneoplastic pemphigus

Citation
A. De Bruin et al., Periplakin and envoplakin are target antigens in canine and human paraneoplastic pemphigus, J AM ACAD D, 40(5), 1999, pp. 682-685
Citations number
15
Categorie Soggetti
Dermatology,"da verificare
Journal title
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
ISSN journal
01909622 → ACNP
Volume
40
Issue
5
Year of publication
1999
Part
1
Pages
682 - 685
Database
ISI
SICI code
0190-9622(199905)40:5<682:PAEATA>2.0.ZU;2-M
Abstract
Background: On the basis of clinical and histopathologic similarities to hu man paraneoplastic pemphigus (PNP), we recently identified the first case o f PNP in a nonhuman species, the dog. Objective: To determine a similar pathogenesis in both species, the present study aimed to define whether common antigens are targeted in dog and man. Methods: Canine and human PNP sera were used in parallel-to immunoprecipita te C-14- labeled human keratinocyte antigens. The immunoreactive proteins w ere then identified by immunoprecipitation of canine keratinocyte extracts with specific antibodies to the antiplakin family members follwed by immuno blot analysis using canine and human PNP sera. Results: Protein bands of 210, 190, 170, and 130 kd were identified in dogs and humans. In both species, envoplakin and periplakin were demonstrated a s antigens. Antidesmoglein 3 antibodies could not be demonstrated in canine PNP, but in human PNP. Conclusion: These results demonstrate that canine PNP closely correlates to the human counterpart and may therefore represent an excellent model for t he human disease.