The effect of hypothermia on the content of 310 kD stress protein in seedlings of winter rye and wheat

The effect of hypothermia on the content of 310 kD stress protein in seedli ngs of winter rye and wheat was studied by rocket-immunoelectrophoresis and radioactive label. The 1-h low-temperature stress was found to result in a n increase in the content of this protein at both above- and below-zero tem peratures. It was found that the increase in the relative content of the pr otein with mel. wt 310 kD, under the effect of short-term low-temperature s tress, occurs due to induction of its synthesis. It has been found that dur ing cold hardening of winter wheat the content of this protein decreases up to 64% compared to the control during the first day of hardening but start s to rise by the third day and reaches 179% by the seventh day, which is we ll correlated with the increase in cold resistance of winter wheat plants d uring cold hardening. (C) 1999 Elsevier Science Ltd. All rights reserved.