M. Alexandrov et al., Fluorescent and electron-microscopy immunoassays employing polyclonal and monoclonal antibodies for detection of goose parvovirus infection, J VIROL MET, 79(1), 1999, pp. 21-32
Polyclonal antibodies (PAbs) raised in geese and eight mice hybridomas secr
eting monoclonal antibodies (MAbs) against the goose parvovirus (GPV) were
prepared. They were used for development of immunofluorescence (IF) and imm
unoelectron-microscopic (IEM) techniques to demonstrate the GPV infection i
n infected organs and biological fluids. The GPV antigens were established
by immunofluorecence within the nuclei and the cytoplasm of many infected c
ells of the chorioallantoic membrane of goose and Peckin duck embryos, live
r and heart of mortally diseased goslings. By means of IEM it was possible
to detect the GPV in native organ homogenate supernatants and allantoic flu
ids. All techniques used in the study could be successfully applied for rap
id diagnosis of the GPV infection. The test systems on the basis of MAbs sh
ould, however, be preferred. By means of immunoblotting (IB) using PAbs and
MAbs four viral proteins (VP) with MW 88, 77, 65 and 60 kDa were demonstra
ted. Contrary to the others the VP with MW 65 kDa was the most antigenicall
y reactive though invisible in the SDS-PAGE and Coomassie-blue dye-stained
preparations. (C) 1999 Elsevier Science B.V. All rights reserved.