Molecular heterogeneity and regulation of poly(ADP-ribose)glycohydrolase

We have recently described the isolation and characterization of bovine cDN A encoding poly(ADP-ribose) glycohydrolase (PARG). We describe here the pre paration and characterization of antibodies to PARG. These antibodies have been used to demonstrate the presence of multiple forms of PARG in tissue a nd cell extracts from bovine, rat, mouse, and insects. Our results indicate that multiple forms of PARG previously reported could result from a single gene. Analysis of PARG in cells in which poly(ADP-ribose) polymerase (PARP ) has been genetically inactivated indicates that the cellular content of P ARG is regulated independently of PARP.