Intracellular trafficking of the JNCL protein CLN3

Juvenile neuronal ceroid lipofuscinosis is a lysosomal storage disease that causes visual impairment, progressive mental deterioration, and eventually death. A predominant 1.02-kb deletion as well as other mutations have been described in the CLN3 gene. Lacking significant identity with proteins of known function and no overt targeting signals within the primary amino acid sequence, accurate predictions of the intracellular location and function could not be made. Further, recent conflicting reports identified CLN3 as e ither a lysosomal or a mitochondrial protein. Transfection experiments usin g native and epitope-tagged fusion proteins were evaluated to help delineat e CLN3 localization. We confirmed by immunohistochemistry and brefeldin A t reatment that NH2-terminal green fluorescence protein (GFP)-CLN3 fusion pro teins were retained in the Golgi apparatus, with no colocalization with mit ochondrial markers. Anti-CLN3 antibodies directed against amino acids 67-90 of CLN3 were generated and shown to be specific for a 50-kDa protein in HE K 293 cells and GFP-CLN3 in transfected cells. However, cells transfected w ith nontagged CLN3 or carboxyl-terminal-tagged CLN3 were not immunoreactive with anti-CLN3 antibodies, suggesting that normally, the amino terminus in teracts with other molecules. Thus, tags on the NH2-terminus probably inhib ited these interactions and movement of CLN3 from the Golgi to more distal compartments. Also, CLN3 tagged at the COOH-terminus with either GFP or FLA G epitopes were retained in the ER, indicating a role for the COOH-terminus in trafficking. Taken together, these data confirm that CLN3 traffics thro ugh the ER and Golgi. (C) 1999 Academic Press.