DETERMINATION OF 10 ORGANOARSENIC COMPOUNDS USING MICROBORE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED WITH ELECTROSPRAY MASS-SPECTROMETRY MASS-SPECTROMETRY
Sa. Pergantis et al., DETERMINATION OF 10 ORGANOARSENIC COMPOUNDS USING MICROBORE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED WITH ELECTROSPRAY MASS-SPECTROMETRY MASS-SPECTROMETRY, Journal of analytical atomic spectrometry, 12(5), 1997, pp. 531-536
An analytical method based on reversed-phase microbore HPLC coupled on
-line with electrospray mass spectrometry (ES-MS) is described, The me
thod allows for the determination of up to ten organoarsenicals in a s
ingle chromatographic run. Excellent sensitivity and selectivity is ac
hieved by operating the triple quadrupole mass spectrometer in the sel
ected-reaction monitoring mode, This is possible because the ions prod
uced by collision-induced dissociation of the protonated molecules or
molecular ions of the ten organoarsenicals are characteristic of each
compound, The best LODs, achieved in the positive-ion mode, were betwe
en 2 and 21 pg of arsenic and corresponded to arsenicals which exist a
s cations in acidic solutions, The selectivity achieved by using this
method allows for successful determination of arsenicals co-eluting du
ring HPLC, This is a major improvement over other hyphenated technique
s already used for arsenic speciation, e.g., HPLC-ICP-MS. Furthermore,
the method was used for the analysis of an undiluted urine SRM in whi
ch arsenobetaine was determined to be present at the low mu g l(-1) le
vel.