Excess nitrate (NO3-) in lakes and streams has deleterious effects for envi
ronmental and human health. Nitrate concentrations have become problematic
in agricultural watersheds due to increased use of fertilizers and improper
management of livestock wastes. Research has indicated that the planting a
nd/or preservation of riparian buffer zones can be an effective means of re
ducing pollution from agricultural fields (Osborne and Kovacic, 1993; Jorda
n et al., 1992; Simmons el al., 1992). Biological denitrification is the mo
st desirable means of nitrate attenuation as the microbial conversion of NO
3- removes nitrate from the watershed in the form of N gases. Despite the i
nherent value of biological denitrification, a comprehensive review discuss
ing the role of this process in removing nitrate from riparian zones is lac
king. In this paper we examine the results and conclusions of past research
on the topic of denitrification in riparian zones and make recommendations
for future research in this area. The need for subsurface denitrification
assays in riparian zones is emphasized.