Schizosaccharomyces pombe stt3(+) is a functional homologue of Saccharomyces cerevisiae STT3 which regulates oligosaccharyltransferase activity

The Saccharomyces cerevisiae STT3 (ScSTT3) gene encodes a protein which is involved in protein glycosylation via the regulation of oligosaccharyltrans ferase activity. We have cloned and isolated the Schizosaccharomyces pombe STT3 homologous gene (Spstt3(+)). The Spstt3(+) gene encodes a protein cons isting of 749 amino acid residues which has significant homology with ScStt 3p and the mouse Stt3p-homologue Itm1p. Disruption of the Spstt3(+) gene sh ows that this gene is essential for growth. Like Itm1, Spstt3(+) partially suppressed the temperature sensitivity of the stt3-1 mutation of S. cerevis iae, indicating that Spstt3(+) is a functional and structural homologue of the ScSTT3 gene. The nucleotide sequence data reported in this paper appear s in the DDBJ/EMBL/GenBank nucleotide sequence database with Accession No. AB015232. Copyright (C) 1999 John Wiley & Sons, Ltd.