Identification of SLPI (secretory leukocyte protease inhibitor) in human mast cells using immunohistochemistry and in situ hybridisation

Authors
Westin, U Polling, A Ljungkrantz, I Ohlsson, K
Citation
U. Westin et al., Identification of SLPI (secretory leukocyte protease inhibitor) in human mast cells using immunohistochemistry and in situ hybridisation, BIOL CHEM, 380(4), 1999, pp. 489-493
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOLOGICAL CHEMISTRY
ISSN journal
14316730 → ACNP
Volume
380
Issue
4
Year of publication
1999
Pages
489 - 493
Database
ISI
SICI code
1431-6730(199904)380:4<489:IOS(LP>2.0.ZU;2-M
Abstract
Recently interest has been focused on secretory leucocyte protease inhibito r (SLPI) and its role in immediate hypersensitive reactions, possibly by in hibiting mast cell chymase. The purpose of this investigation was to show w hether or not SLPI is produced in mast cells. Double-immunolabelling revealed that SLPI coexists with mast cell tryptase (60%) and chymase (37%). On the other hand, in situ hybridisation studies d emonstrated the expression of SLPI mRNA in all mast cells. The differences in results can be attributed to the fact that in site hybridisation is a mo re sensitive method than immunohistochemistry. Hence, we conclude that SLPI is produced in human tonsillar mast cells.