Intrinsic kinetics of biofilms formed under turbulent flow and low substrate concentrations

Reactor operating conditions strongly affect the behaviour of biofilm syste ms, namely their stability and the substrate removal. In this paper, the pe netration of substrate and the activity of biofilms formed by Pseudomonas f luorescens under turbulent flow and low substrate concentrations, are studi ed. A first order diffusion-reaction model was applied to results of biofil m accumulation in steady and non-steady-state. The substrate consumption ra te of the biofilm was calculated based on the on-line determination of the biofilm accumulated on the surface. This approach is important when the res idence time or the substrate concentration on the reactor is very low. Also , the mass transfer of substrate inside the biofilm was measured for every case under study and introduced in the model. The fraction of biofilm penetrated by the substrate depends on the velocity of the fluid that contacts the biological matrix: contrary to biofilms for med at higher velocities, lower velocities give raise to non completely pen etrated biofilms. This fact seems to be associated to the biofilm internal structure in terms of biomass density and compactness of the matrix. They r emove more substrate per reactor volume, but are less resistant from an hyd rodynamic point of view. In conclusion, biofilms formed at higher velocitie s in turbulent flow allow a more stable reactor operation.