Microsatellite DNA as shared genetic markers among conifer species

Polymerase chain reaction (PCR) primer pairs for 21 simple sequence repeat (SSR) loci in Pinus strobus L. and 6 in Pinus radiata D. Don. were evaluate d to determine whether SSR marker amplification could be achieved in 10 oth er conifer species. Eighty percent of SSR primer pairs for (AC), loci that were polymorphic in P. strobus also amplified SSR loci in two other soft pi nes of the subgenus Strobus but not in seven hard pines of the subgenus Pin us, nor in Picea glauca (Moench) Voss or Pseudotsuga menziesii (Mirb.) Fran ce. The six P. strobus SSR primer pairs that did amplify loci from conifers other than soft pines were those that were specific to loci monomorphic wi thin P. strobus. These six loci were also monomorphic within seven other sp ecies tested, but four of the loci were polymorphic among species. A compar ison of allelic variation among the three soft pine species found only 25 s hared alleles among a total of 122 alleles at eight loci. Primer pairs for dinucleotide SSR loci that were polymorphic in Pinus radiata also specifica lly amplified loci from various other hard pines but not from the soft pine s or from the other conifers tested.