ENHANCED RAGE METHOD USING SPECIFIC ENRICHMENT BY BIOTINYLATED OLIGONUCLEOTIDES BOUND TO STREPTAVIDIN COATED MAGNETIC PARTICLES

Authors
LANKIEWICZ S GISSELMANN G HATT H
Citation
S. Lankiewicz et al., ENHANCED RAGE METHOD USING SPECIFIC ENRICHMENT BY BIOTINYLATED OLIGONUCLEOTIDES BOUND TO STREPTAVIDIN COATED MAGNETIC PARTICLES, Nucleic acids research, 25(10), 1997, pp. 2037-2038
Citations number
5
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
25
Issue
10
Year of publication
1997
Pages
2037 - 2038
Database
ISI
SICI code
0305-1048(1997)25:10<2037:ERMUSE>2.0.ZU;2-H
Abstract
RACE (rapid amplification of cDNA ends) is commonly used for identific ation and isolation of 3' and 5' termini of cDNA. We developed an impr ovement of the RACE-method that allows the enrichment of wanted fragme nts. The important new feature is the purification of the amplified pr oducts by biotinylated oligonucleotides that hybridize internally. Hyb rids are isolated by streptavidin coated magnetic particles.