A 12 KB NUCLEOTIDE-SEQUENCE CONTAINING THE ALANINE DEHYDROGENASE GENEAT 279-DEGREES ON THE BACILLUS-SUBTILIS CHROMOSOME

In the framework of the European project aimed at the sequencing of th e Bacillus subtilis genome, a DNA fragment of 12315 bp was cloned and sequenced. The DNA fragment is located between rrnB (275 degrees) and pai (284 degrees). Twelve ORFs were predicted to encode putative prote ins. Two of these (aid and yukI) coincided with known B. subtilis gene s. The products of two other genes (yukK and yukL) showed significant similarity to known proteins present in databases, e.g. pyoverdine syn thase of Pseudomonas aeruginosa and pristinamycine synthase D of Strep tomyces pristinaespiralis.