BRUCELLA-ABORTUS STRAIN-2308 PUTATIVE GLUCOSE AND GALACTOSE TRANSPORTER GENE - CLONING AND CHARACTERIZATION

The gene for the putative transporter for glucose and galactose from B rucella abortus strain 2308 was isolated by functional complementation of Escherichia coli strains lacking either glucose or galactose trans port systems. The same two plasmid clones were isolated from each scre en. These clones restored glucose and galactose transport to the respe ctive E. coli strains. The sequence of the 1806 bp overlap between the se two plasmids was determined. A 1242 bp ORF whose disruption elimina ted complementation of both E. coli strains showed 36% identity with t he E. coli fucP gene encoding a fucose transporter. These two transpor ters are members of the major facilitator superfamily, in which they r epresent a previously undescribed family. In addition, an incomplete g ene similar to E. coli hisG was found. One of the plasmids complemente d E. coli hisG mutants.