Separation of the enantiomers of four chiral drugs by neutral cyclodextrin-mediated capillary zone electrophoresis

Neutral cyclodextrin (CD)-modified capillary zone electrophoresis (CZE) has been applied to the chiral separation of four basic drugs - clorprenaline, benzhexol, esmolol and terazosin. Selector screening and concentration opt imization experiments were performed. The resolution was 3.9 for clorprenal ine, 2.3 for benzhexol, 3.1 for esmolol and 1.2 for terazosin when the runn ing electrolyte was 60 mM hydroxypropyl-beta-CD, 15 mM heptakis (2,3,6-Tri- O-methyl)-beta-CD, 60 mM gamma-CD and 60 mM heptakis (2,6-di-O-methyl)-beta -CD, respectively, in 50 mM, pH 2.5 sodium phosphate buffer.