T. Perez-ruiz et al., Separation and simultaneous determination of quinolone antibiotics by capillary zone electrophoresis, CHROMATOGR, 49(7-8), 1999, pp. 419-423
The potential of capillary zone electrophoresis has been investigated for t
he separation and quantitative determination of some quinolone antibiotics.
The influence of different conditions, such as the nature and concentratio
n of the electrophoretic electrolyte, on migration time, peak symmetry, eff
iciency and resolution was studied. A buffer consisting of 100 mM HEPES adj
usted to pH 8.5 containing 10 % (v/v) acetonitrile was found to furnish a v
ery efficient and stable electrophoretic system for the separation of enoxa
cin, ciprofloxacin, ofloxacin, oxolinic acid, nalidixic acid and pipemedic
acid. A linear relationship between concentration and peak area for each co
mpound was obtained in the concentration range 0.25-40 mu g mL(-1); detecti
on limits were approximately 0.25 ng mL(-1). It was demonstrated that the m
ethod can be used for the simultaneous determination of these six antibioti
cs in serum and urine samples.