Sequencing analysis of forty-eight human image cDNA clones similar to Drosophila mutant protein

We have sequenced 48 human IMAGE cDNA clones selected from the public EST d atabase (dbEST) for their significant homology to Drosophila mutant genes. A dynamically updated analysis report was produced by BlastX and BlastN ana lysis searches in the latest databases available. This analysis led us to e stimate the grade of similarity with homologous genes isolated in other spe cies. Bottlenecks were detected in the sequencing process and here we have presented our problem-solving approach. We think the value of this full-len gth sequencing project is an enrichment of the sequence database informatio n that is currently available to the human genome community.