T. Tang et al., Molecular cloning and characterization of a mouse gene with homology to the Duffy-antigen receptor for chemokines, DNA SEQ, 9(3), 1998, pp. 129-143
The Duffy antigen receptor for chemokines (DARC) is a receptor for both CXC
and CC chemokines. We have cloned a mouse gene with a predicted amino acid
sequence homology of similar to 63% to human DARC and localized this gene
to mouse chromosome 1 between the Xmv41 and D1Mit166 loci. We further demon
strated that, like the human gene, the mouse gene exhibits a single intron
of 462 bp which interrupts the open reading frame between the codons for th
e seventh and eighth amino acid residues. Northern blot analyses revealed t
hat putative mDARC mRNA is highly expressed in adult mouse spleen and skele
tal muscle and in whole embryos between embryonic days 8.5 to 12. Northern
blot analysis of hemangiosarcomas which develop spontaneously in the spleen
of the Eker rat reveal expression of mRNAs which hybridize with both DARC
and CXCR2 probes, suggesting a potential role of these receptors in the ang
iogenesis associated with tumor formation.