LACTOSE-INDUCED EXPRESSION OF BACILLUS SP TS-23 AMYLASE GENE IN ESCHERICHIA-COLI REGULATED BY A T7 PROMOTER

Authors
LIN LL HSU WH
Citation
Ll. Lin et Wh. Hsu, LACTOSE-INDUCED EXPRESSION OF BACILLUS SP TS-23 AMYLASE GENE IN ESCHERICHIA-COLI REGULATED BY A T7 PROMOTER, Letters in applied microbiology, 24(5), 1997, pp. 365-368
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
24
Issue
5
Year of publication
1997
Pages
365 - 368
Database
ISI
SICI code
0266-8254(1997)24:5<365:LEOBST>2.0.ZU;2-Q
Abstract
Bacillus sp. TS-23 amylase gene was amplified by polymerase chain reac tion and cloned into the pET23(+) transcription vector. Lactose induct ion of Escherichia coli BL21(DE3) cells harbouring this recombinant pl asmid resulted in the extracellular production of gene products. By th e addition of 20 mnol l(-1) lactose, the amylase activity of fermentat ion broth had a specific activity of 21 U mg(-1) of protein.