Effect of vitamin E supplementation on post-exercise plasma lipid peroxidation and blood antioxidant status in smokers: with special reference to haemoconcentration effect
E. Surmen-gur et al., Effect of vitamin E supplementation on post-exercise plasma lipid peroxidation and blood antioxidant status in smokers: with special reference to haemoconcentration effect, EUR J A PHY, 79(6), 1999, pp. 472-478
Citations number
40
Categorie Soggetti
Physiology
Journal title
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY
The oxidative effects were investigated of exhausting exercise in smokers,
and the possible protective role of 400 mg.day(-1) vitamin E (Vit E) supple
mentation over a period of 28 days. The subjects exercised to exhaustion in
cluding concentric-eccentric contractions following maximal cycling. The ha
ematocrit and haemoglobin, leucocyte (WBC), plasma lactic acid (La) and mal
ondialdehyde (MDA), erythrocyte superoxide dismutase (SOD) and glutathione
peroxidase (GPx), serum Vit E and ceruloplasmin (CER) concentrations were m
easured pre and post exercise. Supplementation increased Vit E concentratio
ns 28% and 31% in the controls and the smokers, respectively. Cigarette smo
king and/or Vit E supplementation did not influence plasma lipid peroxidati
on or the antioxidant status at rest. Exercise caused significant haemoconc
entration in all groups. When the post-exercise concentrations were adjuste
d for haemoconcentration, a significant elevation in La concentrations due
to exercise was observed in all groups. Similarly, there were significant e
levations in the adjusted WBC counts in all groups except the Vit E supplem
ented controls. The MDA concentrations on the other hand, when adjusted for
haemoconcentration, did not exhibit any difference due to exercise. Exerci
se did not affect the GPx and CER activities either, while causing a SOD ac
tivity loss in all groups except the Vit E supplemented non-smokers. Serum
Vit E concentrations diminished significantly in all groups after exercise.
Postexercise plasma MDA and blood antioxidant concentrations were not alte
red by smoking. The results would suggest that plasma volume changes should
always be taken into account when assessing post-exercise plasma concentra
tions and that smoking and exercise do not have an additional collective ef
fect on plasma lipid peroxidation and the dose of Vit E administered was in
sufficient to maintain the serum concentrations after exercise.