beta-carotene enhances hydrogen peroxide-induced DNA damage in human hepatocellular HepG2 Cells

Citation
Ja. Woods et al., beta-carotene enhances hydrogen peroxide-induced DNA damage in human hepatocellular HepG2 Cells, FEBS LETTER, 449(2-3), 1999, pp. 255-258
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
449
Issue
2-3
Year of publication
1999
Pages
255 - 258
Database
ISI
SICI code
0014-5793(19990423)449:2-3<255:BEHPDD>2.0.ZU;2-5
Abstract
In this study, the alkaline version of the comet assay has been used to det ermine the effect of beta-carotene supplementation (10 mu M) on peroxide-in itiated free radical-mediated DNA damage in human HepG2 hepatoma cells. In supplemented cells, beta-carotene failed to afford any protection against h ydrogen peroxide-induced DNA strand breaks. Indeed, levels of strand breaks in supplemented cells were significantly higher than in cells exposed to h ydrogen peroxide alone, especially after a long incubation period. In contr ast, beta-carotene afforded significant levels of protection against DNA st rand breaks when cells were treated with tert-butyl hydroperoxide, In this case, the level of protection increased as supplementation continued. (C) 1 999 Federation of European Biochemical Societies.