Copia-SSR: A simple marker technique which can be used on total genomic DNA

We have developed a PCR-based assay which uses a combination of two classes of repetitive elements found in eukaryotic genomes, namely Ty1-Copia retro transposons and simple sequence repeats, to amplify multiple polymorphic pr oducts from total genomic DNA. Using the technique, seven markers were mapp ed to four different chromosome arms using a barley doubled haploid mapping population. This technique offers a simple and efficient method of generat ing both dominant and codominant genetic markers in virtually any eukaryote for mapping and diversity studies.