A. Rodriguez-acosta et al., Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection, I J MED RES, 109, 1999, pp. 152-156
We describe the separation of an active glutamate dehydrogenase [GDH (NADP(
+))] enzyme From the plasma of patients with P. falciparum infection using
columns of sepharose anti-GDH (NADP(+)) of Proteus spp. The activity of thi
s enzyme was also detected in P. falciparum culture supernatant. The parasi
tic origin of this enzyme was suggested by western blot analysis using anti
-P. falciparum culture supernatant and anti-whole parasite antibodies. The
differential inhibition of the P. falciparum GDH (NADP(+)) indicates that s
ome epitopes recognised by the antibodies in both preparations may be diffe
rent. The determination of P. falciparum GDH (NADP(+)) activity could be de
veloped into a specific technique for the diagnosis of falciparum malaria.