Single crystals of Bombyx mori silk fibroin in the metastable silk I polymo
rph have been produced using a new foaming technique. Foams of silk protein
are generated by bubbling pure nitrogen gas through an aqueous solution of
regenerated silk fibroin. The foamed material is collected, dried, and the
n sonicated to yield individual crystals which were examined using transmis
sion electron microscopy and electron diffraction. It is found that slightl
y acidic conditions in the solution from which the foam was generated favor
the formation of silk II while neutral to slightly basic solutions favor s
ilk I formation. More dilute solutions favor the formation of silk II while
more concentrated solutions (about 7 wt.% or greater) favor the formation
of silk I. X-ray powder diffraction patterns from the dried silk I foams di
splayed features highly indicative of silk I. We also report the first sing
le crystal electron diffraction patterns of silk I. These patterns indicate
a large unit cell, possibly 22.66 x 5.70 x 20.52 Angstrom, with six chains
of six residues, Gly-Ala-Gly-Ala-Gly-Ser. Although we have not fully chara
cterized this complex structure it appears that the chain is nearly fully e
xtended and thus our data is consistent with models possessing general feat
ures similar to those proposed by Fossey SA, Nemethy G, Gibson KD, Scheraga
HA. (Biopolymers 1991;31:1529-1541). (C) 1999 Elsevier Science B.V. All ri
ghts reserved.